Molecular Differentiation of Renibacterium salmoninarum Isolates from Worldwide Locations

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Molecular differentiation of Renibacterium salmoninarum isolates from worldwide locations.

Renibacterium salmoninarum is a genospecies that is an obligate pathogen of salmonid fish and is capable of intracellular survival. Conventional typing systems have failed to differentiate isolates of R. salmoninarum. We used two methods to assess the extent of molecular variation which was present in isolates from different geographic locations. In one analysis we investigated possible polymor...

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Molecular diversity of Renibacterium salmoninarum isolates determined by randomly amplified polymorphic DNA analysis.

The molecular diversity among 60 isolates of Renibacterium salmoninarum which differ in place and date of isolation was investigated by using randomly amplified polymorphic DNA (RAPD) analysis. Isolates were grouped into 21 banding patterns which did not reflect the biological source. Four 16S-23S rRNA intergenic spacer (ITS1) sequence variations and two alleles of an exact tandem repeat locus,...

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Further characterization of Renibacterium salmoninarum extracellular products.

Renibacterium salmoninarum, the agent of bacterial kidney disease in salmonids, releases high concentrations of extracellular protein in tissues of infected fish. The extracellular protein consists almost entirely of a 57-kDa protein and derivatives of degradation and aggregation of the same molecule. The 57-kDa protein and its derivatives were fractionated into defined ranges of molecular mass...

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Flavobacterium psychrophilum, Renibacterium salmoninarum and Aeromonas salmonicida

Intra-ovum infection of artificialy contaminated salmonid (rainbow trout or amago salmon) eggs was studied using three fish pathogens (Flavobacterium psychrophilum, Renibacterium salmoninarum and Aeromonas salmonicida). Artificial contamination was performed using egg-surface contamination or immersion-water contamination. For F. psychrophilum and R. salmoninarum, infection occurred by the entr...

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Genetic Variation of Renibacterium salmoninarum Genes in Infected Salmonids

I understand that my project will become part of the permanent collection of Oregon State University, Bioresource Research. My signature below authorizes release of my project to any reader upon request. Abstract Renibacterium salmoninarum is the causative agent of bacterial kidney disease in both wild and farmed salmonid species worldwide. The genome of this pathogen has significant synteny to...

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ژورنال

عنوان ژورنال: Applied and Environmental Microbiology

سال: 1999

ISSN: 0099-2240,1098-5336

DOI: 10.1128/aem.65.3.961-968.1999